Variable DNA content of <Emphasis Type="Italic">Cyclamen persicum</Emphasis> regenerated via somatic embryogenesis: rethinking the concept of long-term callus and suspension cultures |
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| Authors: | Thomas Borchert Jörg Fuchs Traud Winkelmann Annette Hohe |
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| Institution: | 1.Department of Hospital and Clinical Engineering, Environment and Biotechnology,University of Applied Sciences Giessen,Giessen,Germany;2.Department of Plant Propagation,Institute of Vegetable and Ornamental Crops,Erfurt-Kuehnhausen,Germany;3.Leibniz-Institute of Plant Genetics and Crop Plant Research,Gatersleben,Germany;4.Institute of Floriculture and Woody Plant Science,Leibniz Universit?t Hannover,Hannover,Germany;5.University of Applied Sciences Weihenstephan,Freising,Germany |
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| Abstract: | By flow cytometric experiments in Cyclamen persicum both with propidium iodide (PI) and DAPI (4′,6-Diamidino-2-phenylindol)-staining we were able to present (1) a new estimation
for the absolute DNA content in the range of 3.17 pg DNA/2C and (2) ploidy abnormalities which were detected in the pathway
of somatic embryogenesis. These aberrations might have arisen from ploidy mutations or abnormal polyploidization processes.
Morphologically normal somatic embryos contained the smallest proportion of individuals with ploidy abnormalities, but also
a regenerated plant in the green house displayed a significantly elevated DNA content. The results are discussed in the framework
of future application of in vitro propagation techniques based on regeneration from fastly growing undifferentiated cells
like long-term callus and suspension cultures. |
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| Keywords: | Flow cytometry DNA amount DNA-Index Micropropagation Somaclonal variation |
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