Patch-clamp study on membrane properties and transmitter activated currents of rabbit area postrema neurons |
| |
| Authors: | J. Bufler A. Weindl T. Arzberger K. Jahn H. Hatt |
| |
| Affiliation: | (1) Neurologische Klinik der Technischen Universität München, Möhlstrasse 28, D-81675 München, Germany;(2) Physiologisches Institut der Technischen Universität München, Biedersteinerstrasse 29, D-80802 München, Germany;(3) Present address: Lehrstuhl für Zellphysiologie, Universitätsstrasse 150, Ruhr-Universität Bochum, D-44780 Bochum, Germany |
| |
| Abstract: | Using the patch-clamp technique in combination with sliced tissue preparation the membrane properties of newborn rabbit area postrema neurons were investigated. The neurons responded upon depolarization with a fast Na +-current followed by an inactivating and non-inactivating K +-current. GABA-activated currents were investigated resulting in a large Cl--conductance, indicating the expression of GABAA-receptors. The expression of glutamate receptor mRNA was studied by in situ hybridization and electrophysiological measurements of these receptors by means of the patch-clamp technique. As a main result it was found that ionotropic glutamate receptors in the area postrema are composed of  flop variants of the GluA-, GluB- and GluC-subunits.Abbreviations AP area postrema - GABA  -aminobutyric acid - Glu glutamate - I–V current-voltage - SFO subfornical organ |
| |
| Keywords: | Area postrema Rabbit Patch clamp Glutamate-receptor GABA-receptor |
| 本文献已被 SpringerLink 等数据库收录！ |
|
