A fluorescence microscope attachment for flow-through cytofluorometry |
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Authors: | G von Sengbusch B Hugemann |
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Affiliation: | Battelle-Institut e. V., Frankfurt am Main, BRD |
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Abstract: | A flow-through system for rapid automated analysis of intracellular fluorescence parameters is described. In combination with commercially available instrumentation for fluorescence microscopy, fluorometry and pulse height analysis, the hydrodynamic focusing flow-through principle can be successfully applied. The specific advantages and disadvantages of this principle compared with the mechanical focusing system are discussed. |
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