腐乳毛霉蛋白酶菌株紫外线诱变育种

目的：选育酶活力高、耐热性好的毛霉蛋白酶菌株,用于腐乳发酵实验或生产。方法：从腐乳中分离毛霉蛋白酶菌株,对其出发菌株进行紫外线诱变,并经控温培养,选育优良变株。结果：获得一株酶活力高、耐热性强的优良变株SCLG—毛霉16,该菌株在32℃条件下,生长茂盛,其HE值为1.39、蛋白酶活力为180.375u/g,分别较出发菌株高出7.8%和51.4%。结论：将此优良变株用于腐乳发酵实验或生产,在缩短发酵时间,提升腐乳质量,延长生产季节等方面具有一定的应用价值。

UV Breeding by Induced Mutation of Mucor Protease Strain from Sufu

Objective:Breeding of Mucor protease strain with high enzyme activity and good high-temperaturre for fermentation experiments or production of sufu.Method: Mucor protease strains were picked out from sufu,its original strain was induced mutation by UV and fostered by temperature control,breeding of excellent mutant strain.Result:One high enzyme vitality and excellent high-temperature mutant strain named SCLG-mucor 16 was selected,the strain can strongly grow at 32℃.Its HE is 1.39,protease vitality is 180.375u/g,which was enhanced by 7.8% and 51.4% more than the original strain respectively.Conclusion: The excellent mutant strain was used for fermentation experiments or production of sufu, it has a certain using value in shortening the fermentation time,enhancing the quality of sufu and extending the season of production.