| 神经病理痛大鼠DRG神经元GABAA受体激活电流的变化 |
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| 引用本文： | Zhu H,Ma KT,Li L,Zhang ZS,Li J,Si JQ. 神经病理痛大鼠DRG神经元GABAA受体激活电流的变化[J]. 中国应用生理学杂志, 2011, 27(3): 376-379 |
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| 作者姓名： | Zhu H Ma KT Li L Zhang ZS Li J Si JQ |
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| 作者单位： | 新疆民族高发病教育部重点实验室;石河子大学医学院生理教研室;武汉大学基础医学院; |
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| 基金项目： | 石河子大学高层次人才项目(RCZX200792) |
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| 摘 要： | 目的：观察坐骨神经慢性压榨损伤（CCI）致神经病理痛后,大鼠背根节神经元GABAA受体（γ-氨基丁酸A受体）激活电流的变化。方法：运用全细胞膜片钳技术记录CCI模型手术侧、手术对侧及假手术组大鼠背根神经节细胞GABAx受体激活电流,比较坐骨神经慢性压榨损伤后GABAA受体激活电流的变化。结果：①CCI模型组大鼠手术侧DRG神经元在不同浓度（0．1-1000μmol／L）GABAA受体激活电流幅值均显著小于假手术组。②CCI模型组大鼠手术对侧DRG神经元在不同浓度（0．01-1000μmol／L）GABAA受体激活电流幅值均显著大于手术同侧及假手术组。结论：在坐骨神经慢性压榨损伤的过程中,不仅损伤侧的DRG神经元GABAA受体激活电流显著减小,这种损伤同时还引起了手术对侧的DRG神经元GABA激活电流代偿性的增强,GABAA受体功能的改变导致的突触前抑制作用的减弱可能是神经病理痛产生的根本原因之一。
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| 关 键 词： | 神经病理痛 背根神经节 γ-氨基丁酸受体 全细胞膜片钳 |
Changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain |
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| Zhu He,Ma Ke-tao,Li Li,Zhang Zhong-shuang,Li Jing,Si Jun-qiang. Changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain[J]. Chinese journal of applied physiology, 2011, 27(3): 376-379 |
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| Authors: | Zhu He Ma Ke-tao Li Li Zhang Zhong-shuang Li Jing Si Jun-qiang |
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| Affiliation: | ZHU He1,2,MA Ke-tao1,LI Li1,ZHANG Zhong-shuang1,LI Jing1,SI Jun-qiang1,3(1.The key Laboratory of Xinjiang Endemic and Ethnic Diseases,2.Department of Physiology,Shihezi University Medical College,Shihezi 832002,China3.Department of Physiology,Wuhan University School of Basic Medical Sciences,Wuhan 430070,China) |
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| Abstract: | Objective： To investigate the changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathie pain. Methods： The neuropathie pain model was established by chronic eonstrietion injury（CCI） 7 days before eleetrophysiolngiealrecording. The rat DRG neurons were enzymatieally dissociated. Whole-cell patch clamp technique was used to record GABA-activated currents. The changes of cmrents of injured side and opposite side were expected to compare with control group. Results： （1）The currents of injured side of CCI group were notablely decreased compared with control group（GABA concentration, 0.1-1 000 μ mol/L）. （2） By the contrast, opposite side currents of CCI group increased significantly compared with those in injured side and control group（GABA concentration, 0.01-1 000 μmol/L）. Conclusion： The data indicates that the chronic constriction injury change both the function of GABAA receptors of injury side and opposite side. The decrease of pre-synaptic inhibition of GABA may be the passible reason of neuropathic pain. |
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| Keywords: | neuropathic pain dorsal root ganglion(DRG) GABA whole-cell patch clamp |
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