Identification and inter-relationship analysis of Bradyrhizobium japonicum strains by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) |
| |
| Authors: | V. R. Lunge N. Ikuta A. S. K. Fonseca D. Hirigoyen M. Stoll S. Bonatto L. S. Ozaki |
| |
| Affiliation: | (1) Centro de Biotecnologia-UFRGS, Av Bento Gonçalves, 9500, Caixa Postal 15.005, CEP 91.501-970 Porto Alegre, Rio Grande do Sul, Brazil;(2) Depto Citogenética, Instituto de Investigaciones Biológicas Clemente Estable, Casilla Correo 16012, C.P. 11.600 Montevideo, Uruguay;(3) Depto Genética-UFRGS, Av Bento Gonçalves, 9500, Porto Alegre, Rio Grande do Sul, Brazil |
| |
| Abstract: | ![]()
Genomic DNA of 13 Bradyrhizobium japonicum strains was prepared and analysed by restriction fragment length polymorphism (RFLP) with nif and nod probes, and by random amplified polymorphic DNA (RAPD) with 11 primers of arbitrary nucleotide sequence. Polymorphism was observed in both analyses. The RFLP and RAPD banding patterns of different strains were used to calculate genetic divergence and to construct phylogenetic trees, allowing studies on the relationships between the strains. RFLP with nif and nod probes permitted the separation of the strains into two divergent groups, whereas RAPD separated them into four main groups. RAPD allowed closely related strains to be distinguished. |
| |
| Keywords: | Bradyrhizobium japonicum nitrogen fixation RAPD RFLP strain identification |
| 本文献已被 SpringerLink 等数据库收录！ |
|
