In vitro culture and plant regeneration of large flowered purslane |
| |
| Authors: | Badr-Din Rossi-Hassani Jean-Pierre Zryd |
| |
| Institution: | (1) Laboratoire de Phytogénétique Cellulaire, Université de Lausanne, 1015 Lausanne, Switzerland;(2) Present address: Laboratoire de Biochimie & Biologie Moléculaire, Faculté des Sciences-Dhar Mehraz, Fès B.P. 1796, Morocco |
| |
| Abstract: | Culture conditions were established for callus induction from a range of Portulaca grandiflora Hook tissues. Rapidly growing calli were obtained on Murashige and Skoog medium with stem-, leaf- and sepal-derived explants. Plant regeneration via organogenesis was explant-origin dependent with hypocotyl tissues giving the highest shooting frequency. Light conditions, pH and carbon source had a pronounced effect on the percentage of explants regenerating buds and the number of buds formed. It was possible to establish stable regenerated plants in the glasshouse.Abbreviations BA
6-benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
1-naphthaleneacetic acid
- IAA
indoleacetic acid
- MS
Murashige and Skoog (1962) medium |
| |
| Keywords: | callus culture hypocotyl regenerants Portulaca grandiflora |
| 本文献已被 SpringerLink 等数据库收录！ |
|
