Embryo sac development is affected in Petunia inflata plants transformed with an antisense gene encoding the extracellular domain of receptor kinase PRK1 |
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Authors: | H-S Lee Y-Y Chung Chandreyee Das B Karunanandaa Jacques L van Went Celestina Mariani T-H Kao |
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Institution: | (1) Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802–4500, USA; Tel. +1–814–863–1042; Fax 1–814–863–9416 e-mail txk3@psu.edu, US;(2) Department of Experimental Botany, Catholic University of Nijmegen, Toernoiveld 1, 6525 ED Nijmegen, The Netherlands, NL;(3) Department of Plant Cytology and Morphology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands, NL |
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Abstract: | In a previous study of the function of a pollen-expressed receptor kinase of Petunia inflata, PRK1, it was found that transgenic plants carrying an antisense-PRK1 gene were unable to transmit the transgene through either the male or, unexpectedly, the female. In this report, the nature
of this female phenotype was studied using one of the transgenic plants, ASRK-13. Electron and light microscopic examination
of the embryo sac and seed development of ASRK-13 and a wild-type plant revealed that embryo sac development of approximately
half of the ovules of ASRK-13 was abnormal. The development of the affected embryo sacs was arrested at the late stages of
megagametogenesis. The majority of the affected embryo sacs completed three rounds of mitosis normally, but failed to progress
through the maturation stages when cell expansion, nuclear migration, and differentiation take place. The remaining small
number of abnormal embryo sacs were arrested at either the four- or eight-nucleate stages. The ovules containing the defective
embryo sacs apparently failed to be fertilized, resulting in degeneration of half of the seeds produced by ASRK-13. RNA gel
blot analysis suggests that the PRK1 gene is expressed in the ovary, albeit at a much lower level than in the anther. The possibility that the antisense PRK1 gene is responsible for the abnormal embryo sac development is discussed.
Received: 25 April 1997 / Revision accepted: 25 June 1997 |
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Keywords: | Embryo sac development Ovule Petunia inflata Receptor kinase |
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