Formation of antigenic extracellular polysaccharides by selected strains ofMucor spp.,Rhizopus spp.,Rhizomucor spp.,Absidia corymbifera andSyncephalastrum racemosum |
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| Authors: | Gerhard A De Ruiter Andrea W Van Bruggen-van der Lugt M J Robert Nout Wouter J Middelhoven Pop S S Soentoro Servé H W Notermans Frank M Rombouts |
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| Institution: | (1) Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD Wageningen, The Netherlands;(2) Department of Microbiology, Wageningen Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands;(3) Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA Bilthoven, The Netherlands |
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| Abstract: | In this study, polyclonal IgG antibodies raised against extracellular polysaccharides (EPS) ofMucor racemosus were characterised as almost specific for moulds belonging to the order of Mucorales. Cross-reactivity in the ELISA could be observed only towards the yeastPichia membranaefaciens. EPS were isolated from various cultures ofM. hiemalis growing on six different carbon sources and two nitrogen sources, with ratios varying from 0.13 to 0.44 relative to the amount of biomass. Other strains includingMucor spp.,Rhizopus spp.,Rhizomucor spp.,Absidia corymbifera andSyncephalastrum racemosum also excreted EPS, with ratios varying from 0.05 to 0.23. In all cases, the excreted EPS had similar antigenic properties as determined by ELISA. No enzymatic degradation of the antigenic parts of the polysaccharides could be observed upon prolonged incubation. Considering that all tested strains formed similar amounts of antigenic EPS there might be scope for the specific detection of biomass of Mucoralean moulds using ELISA techniques for example in food. |
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| Keywords: | antigen ELISA extracellular polysaccharides Mucor Pichia Rhizopus |
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