Tissue Fixation for Immunohistochemical Detection of Proliferating Cell Nuclear Antigen with PC10 Monoclonal Antibody |
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| Authors: | Andrea Casasco Marco Casasco Antonia Lcaro Cornaglia Marco Danova Monica Giordano Alberto Calligaro |
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| Institution: |
a Institute of Histology and Embryology, University of Pavia, Pavia, Italy
b Department of Internal Medicine, Section 2nd Medical Clinic I.R.C.C.S, San Matteo Hospital, Pavia, Italy |
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| Abstract: | PC10 is a monoclonal antibody to proliferating cell nuclear antigen, a nuclear protein associated with the cell cycle. We have evaluated the effects of tissue fixation on PC10 immunoreactivity in sections of paraffin embedded rat tissues. Immunoreactivity was well preserved in tissues after fixation with alcohol-based solutions for 3-24 hr. Fewer PC10-positive cells were detectable in samples fixed with formaldehydecontaining solutions compared with samples fixed with alcohol for the same time. Loss of PC10 immunoreactivity in formaldehyde fixed tissues was progressive, and quantifiable as early as after 3 hr fixation. Consequently, alcohol-based fixatives are strongly recommended for any immunocytochemical prospective study using PC10 antibody. In contrast, loss of PC10-immunoreactivity is always predictable, but difficult to quantitate, using formaldehyde fixed specimens. This aspect should be considered when using PC10 antibody in retrospective studies with routinely-processed archival material. |
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| Keywords: | proliferating cell nuclear antigen cyclin replicating cell cell proliferation PC10 monoclonal antibody Fixation immunohistochemistry |
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