应用动态光散射对一些蛋白质结晶性能的研究

动态光散射是研究生物大分子溶液物化行为和结晶性能的重要工具。以溶菌酶为模型蛋白 ,对其溶液的DLS研究表明 ,一定范围的蛋白质浓度不会影响蛋白质聚合性质 ,而起沉淀剂作用的盐对多色散性的影响较大。考虑这些研究结果 ,应用DLS研究对空间微重力下晶体生长样品的质量做了考察 ,这对于提高空间样品准备水平 ,进而提高空间实验成功率是一重要途径。

DYNAMIC LIGHT SCATTERING STUDIES OF CRYSTALLIZATION PROPERTIES OF SOME PROTEINS

Dynamic light scattering (DLS)is a powerful tool to study properties of biological macromolecules in solutions. DLS studies of lysozyme,as model protein, have shown that the protein concentration in a certain rangs does not influence the aggregation property obviously, but the salt, acting as preciptant, may affect the polydispersity greatly. Taking these results into account, the sample quality was investigated for some proteins to be used in space crystallization experiments. This may be a good way to improve samples in order to increase the success rate of protein crystallzation in space.