| 红色荧光蛋白的光谱多样性及体外分子进化 |
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| 引用本文： | 樊晋宇,崔宗强,张先恩.红色荧光蛋白的光谱多样性及体外分子进化[J].生物化学与生物物理进展,2008,35(10):1112-1120. |
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| 作者姓名： | 樊晋宇 崔宗强 张先恩 |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院,武汉,430074;中国科学院武汉病毒研究所,病毒学国家重点实验室,武汉,430071
2. 中国科学院武汉病毒研究所,病毒学国家重点实验室,武汉,430071 |
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| 摘 要： | 从珊瑚中来源的各种红色荧光蛋白（red fluorescent protein，RFP）经过一系列体外进化，其波谱范围覆盖了570－655mn，极大地丰富了细胞内或体内光学成像的荧光探针．简要阐述了来源于Discosoma sp．，Entacmaea quadricolor,Anemonia sulcata,Heteractis crispo,Actinia equina5种珊瑚的红色荧光蛋白的光学特征、结构、体外分子进化及其应用．
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| 关 键 词： | 红色荧光蛋白 分子进化 光学性质 |
| 收稿时间： | 2008/2/12 0:00:00 |
| 修稿时间： | 2008/5/11 0:00:00 |
Optical Spectra Diversity and in vitro Molecular Evolution of Red Fluorescent Proteins |
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| FAN Jin-Yu,CUI Zong-Qiang and ZHANG Xian-En.Optical Spectra Diversity and in vitro Molecular Evolution of Red Fluorescent Proteins[J].Progress In Biochemistry and Biophysics,2008,35(10):1112-1120. |
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| Authors: | FAN Jin-Yu CUI Zong-Qiang and ZHANG Xian-En |
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| Institution: | College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China;State Key Laboratory of Virology, Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan 430071, China;State Key Laboratory of Virology, Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan 430071, China;State Key Laboratory of Virology, Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan 430071, China |
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| Abstract: | Red fluorescent proteins (RFPs) produced from a number of Anthozoa species have been subjected to a series of in vitro molecular evolution, resulting in various emission spectra ranging from 570 nm to 655 nm and thus providing powerful tools for cellular imaging or even body imaging. This article briefly reviewed the optical properties, structures and mutagenesis of RFPs and their applications. |
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| Keywords: | red fluorescent protein molecular evolution optical property |
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