Effect of soya lecithin on the enzymatic system of the white-rot fungi Anthracophyllum discolor |
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Authors: | M Bustamante M E González A Cartes and M C Diez |
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Institution: | (1) Doctoral Program in Sciences of Natural Resources, University of La Frontera, Ave. Francisco Salazar 01145, Box 54-D, Temuco, Chile;(2) Department of Chemical Engineering, University of La Frontera, Ave. Francisco Salazar 01145, Box 54-D, Temuco, Chile;(3) Department of Chemical Engineering, Environmental Biotechnology Center, Faculty of Engineering, Sciences and Administration, University of La Frontera, Ave. Francisco Salazar 01145, Box 54-D, Temuco, Chile |
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Abstract: | The present work optimized the initial pH of the medium and the incubation temperature for ligninolytic enzymes produced by
the white-rot fungus Anthracophyllum discolor. Additionally, the effect of soya lecithin on mycelial growth and the production of ligninolytic enzymes in static batch
cultures were evaluated. The critical micelle concentration of soya lecithin was also studied by conductivity. The effects
of the initial pH (3, 4, and 5) and incubation temperature (20, 25, and 30°C) on different enzymatic activities revealed that
the optimum conditions to maximize ligninolytic activity were 26°C and pH 5.5 for laccase and manganese peroxidase (MnP) and
30°C and pH 5.5 for manganese-independent peroxidase (MiP). Under these culture conditions, the maximum enzyme production
was 10.16, 484.46, and 112.50 U L−1 for laccase, MnP, and manganese-independent peroxidase MiP, respectively. During the study of the effect of soya lecithin
on A. discolor, we found that the increase in soya lecithin concentration from 0 to 10 g L−1 caused an increase in mycelial growth. On the other hand, in the presence of soya lecithin, A. discolor produced mainly MnP, which reached a maximum concentration of 30.64 ± 4.61 U L−1 after 25 days of incubation with 1 g L−1 of the surfactant. The other enzymes were produced but to a lesser extent. The enzymatic activity of A. discolor was decreased when Tween 80 was used as a surfactant. The critical micelle concentration of soya lecithin calculated in our
study was 0.61 g L−1. |
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