| 长孢被孢霉利用淀粉质原料发酵产微生物油脂 |
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| 引用本文： | 赖婧,;张波,;姚日生.长孢被孢霉利用淀粉质原料发酵产微生物油脂[J].生物加工过程,2014(4):16-19. |
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| 作者姓名： | 赖婧 ;张波 ;姚日生 |
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| 作者单位： | [1] 合肥工业大学 医学工程学院,合肥230009; [2] 合肥工业大学 化学工程学院,合肥230009; [3]农产品生物化工教育部工程研究中心,合肥 230009 |
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| 基金项目： | 国家高技术研究发展计划（863计划）（2014AA021902） |
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| 摘 要： | 通过研究长孢被孢霉(Mortierella elongate)的发酵过程,并采用摇瓶分批补料发酵模式考察了起始补料时间及补料基质对长孢被孢霉合成微生物油脂的影响。结果发现该菌株合成油脂主要在发酵48~96 h进行,且N源对菌株的生长有促进作用,采用限氮补料发酵可大幅度提高微生物油脂的产量。最适培养条件:可溶性淀粉20 g/L,玉米浆3 g/L,补料起始时间为发酵48 h,单次补加可溶性淀粉4 g。在此条件下,油脂产量较不补料时增加了521.74 mg,增长率为237.1%。
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| 关 键 词： | 分批补料 微生物油脂 长孢被孢霉 可溶性淀粉 |
Fermentation of microbial lipid by Mortierella elongate with starch |
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| Institution: | LAI Jing ,ZHANG Bo, YAO Risheng ( 1. School of Medical Engineering, Hefei University of Technology, Hefei 230009, China; 2. School of Chemical Engineering, Hefei University of Technology, Hefei 230009, China ; 3. Engineering Research Center of Bioproeess of the Ministry of Education, Hefei 230009, China) |
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| Abstract: | The fermentation process of Mortierella elongate and a fed-batch strategy were investigated.The results showed that the synthesis of lipid was conducted during 48 h to 96 h,and nitrogen sources could promote the growth of the strains.Nitrogen-limited fed-batch process improved the production of lipid.The optimum concentration of carbon and nitrogen sources was 20 g/L,and 3 g/L,respectively.Under these conditions,feeding of 4 g soluble starch at the third day of the fermentation could increase the lipid by 521.74 mg with a growth rate of 237.1%. |
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| Keywords: | fed-batch microbial lipid Mortierella elongate soluble starch |
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