益坤宁对围绝经期大鼠卵巢bcl-2和bax表达的影响

目的:研究中药益坤宁(Yikunning,YKN)对围绝经期大鼠卵巢bcl-2和bax基因表达的影响,探讨益坤宁治疗围绝经期综合征的作用机制。方法:选用自然衰老的围绝经期雌性大鼠,随机分为3组(n=10):围绝经期对照组、利维爱(Livial)对照组和益坤宁组,另选10只青年雌性大鼠作为青年对照组。连续灌胃处理4周后,采用逆转录-聚合酶链反应(RT-PCR)检测大鼠卵巢中凋亡相关基因bcl-2和baxmRNA表达,采用蛋白印迹(Westernblot)检测大鼠卵巢中Bcl-2和Bax蛋白表达。结果:益坤宁组大鼠卵巢中Bcl-2、BaxmRNA及其蛋白表达高于围绝经期对照组,差异有统计学意义(P0.01);益坤宁组大鼠卵巢中Bcl-2/BaxmRNA比值和蛋白比值高于围绝经期对照组,差异有统计学意义(P0.05或P0.01)。结论:中药益坤宁通过上调围绝经期大鼠卵巢中凋亡相关基因bcl-2和bax的表达,上调Bcl-2/BaxmRNA比值和蛋白比值,从而延缓卵巢衰老,这可能是其治疗围绝经期综合征的分子机制之一。

Effects of Yikunning on the expressions of bcl-2 and bax in rat ovaries during perimenopausal period

Objective：To investigate the effects of Yikunning（YKN,Chinese Traditional Medicine） on the expressions of bcl-2 and bax in rat ovaries during perimenopausal period.Methods：Thirty female Wistar rats during perimenopausal period were selected by unforced aging.Then the rats were divided into 3 groups randomly：YKN group,Livial control group and Aged control group.Ten young female Wistar rats were selected as young control group.Intragastric administrations were conducted for 4 weeks once daily continuously.The expressions of Bcl-2 Bax mRNAs and proteins in rat ovaries were detected by RT-PCR and Western blot.Results：The levels of Bcl-2,Bax mRNAs and proteins in rat ovaries in YKN group were higher than those in Aged control group,which showed differences among them（P0.01）.The Bcl-2/Bax mRNA rate and protein rate in rat ovaries in YKN group were higher than those in Aged control group,which showed differences among them（P0.05 or P0.01）.Conclusion：YKN could increase the expressions of Bcl-2,Bax mRNAs and proteins and up-regulate the Bcl-2/Bax mRNA rate,protein rate in rat ovaries during perimenopausal period,which might be one of the molecular mechanisms of YKN postponed the ovarian failure and cured perimenopausal syndrome.