采用OCT系统对组织光学通透的研究

组织通透方法采用高折射率化学试剂对生物组织进行渗透,改变组织的光学均匀性,可以有效地改善光学成像的穿透深度,受到生物医学光学研究领域的重视。利用光学相干层析成像技术,测量通透过程中不同测量深度下组织的散射特征的变化。通过采用系统信号对数的梯度值近似地表征光学散射系数,研究了通透过程中组织的散射特征随渗透时间和测量深度的动态关系。实验证明了组织通透可以有效地增加光子的穿透深度,并改善成像质量。研究发现:不同测量深度处组织的散射系数及其变化幅度、变化过程和变化趋势等均存在一定的差异性,并与组织的微观结构、其通透效果,化学试剂在组织中的渗透行为等有密切关系,有助于组织通透过程的理解,并为组织通透机制提供可能的实验依据。

A Study of the Optical Clearing by OCT

Optical clearing can effectively control the optical properties of in tissue using the optical immersing technique by hyperosmotic agents and be paid more and more attention in the biomedical and optical field.This mothed could reduce the number of the multiple scattered photons,increase the depth of the optical imaging,and get a better contrast grade of images.We measured the change of the optical properties of the tissue of the different depth during the optical clearing process using optical coherence tomography(OCT).We applied the gradient of the OCT signal algorithm to analyze the temporal and spatial distribution of the scattering coefficient during the clearing process.It was demonstrated experimentally that optical clearing could effectively improve the OCT imaging depth.During the process of the optical clearing,the scattering coefficient,the range and the time and the trend of the change of the scattering coefficient changed with the different depth,which was related to the tissue microstructure,the clearing effect due to the microstructure and the diffuse process of the agent.The results could be used to understand the process and the mechanism of the optical clearing.