An isozyme-specific selective system for the recovery of mammalian cells deficient in hepatic alcohol dehydrogenase activity |
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| Authors: | A M Killary R E K Fournier |
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| Institution: | Department of Microbiology and the Comprehensive Cancer Center, University of Southern California School of Medicine, Los Angeles, CA 90033, USA |
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| Abstract: | A selective system toxic towards mammalian cells expressing the liver-specific isozyme of alcohol dehydrogenase (L-ADH) has been developed. A number of alpha-unsaturated primary and secondary alcohols were assayed for their ability to serve as substrates for rat liver ADH and were screened for cytotoxicity towards L-ADH+ and L-ADH- cells. 1-Propen-3-ol and 1-penten-3-ol were identified as agents showing selective cytotoxicity. Reconstruction experiments demonstrated that 1-propen-3-ol at a concentration of 15 microM could be used to recover L-ADH- clones from mixed populations of L-ADH+ and L-ADH cells. Cells expressing the non-allelic S-ADH isozyme were not killed under these conditions. The selective system defined in this report is thus isozyme-specific. |
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| Keywords: | To whom offprint requests should be sent Address: Harvard Medical School Department of Physiology and Biophysics 25 Shattuck Street Boston MA 02115 USA |
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