淋巴细胞发育相关基因在鼻腔NK/T细胞淋巴瘤中表达的研究

探索鼻腔NK/T细胞淋巴瘤(N-NK/T-L)中淋巴细胞发育相关基因的表达及意义。方法:通过对EOMEs,ETS-1和MEF基因进行克隆、探针制备和组织芯片制备,对25例N-NK/T-L以及同部位11例B细胞淋巴瘤(BCL)、6例T细胞淋巴瘤(TCL)、3例正常脾组织和5例慢性炎性鼻黏膜组织进行了原位杂交检测。结果:EOMES、ETS-1、MEF基因在N-NL/T-L、BCL、TCL、脾和鼻粘膜中均有表达,但阳性率和表达强度不同。EOMES基因在N-NK/T-L的表达强度与BCL和TCL间有差异,MEF基因在N-NK/T-L的表达阳性率和强度与TCL间有差异,而ETS-1基因在N-NK/T-L与对照组间的表达无差异。T-bet、EOMES和MEF基因在N-NK/T-L的表达具有相关性。结论EOMES和MEF基因在N-NK/T-L中高表达,可能在该肿瘤发生和组织坏死中起重要作用,而ETS-1因在N-NK/T-L和对照组中普遍表达,提示其可能在淋巴造血系统的发育和功能中起基础性的共同通路。

Expression and Significance of the Genes Involved in Lymphoid Development in Nasal NK/T-Cell Lymphoma

To explore the expression and significance of the genes related to lymphocyte development in nasal NK/T lymphoma (N-NK/T-L).Methods:The expression of EOMES(eomesodermin homolog)gene,ETS-1 gene and MEF (myeloid Elf-1 like factor) gene in 25 cases of N-NK/T-L and 25 control cases (including 11 cases of B cell lymphoma (BCL), 6 cases of T cell lymphoma(TCL),3 cases of normal spleen, 5 cases of chronic nasopharyngifis) were detected by tissue microarray and in situ hybridization.Results:Three genes each have its expression in N-NK/T-L and control groups.EOMES gene expressed in all N-NKdT-L and 840/0 of them were high expression.Compared with BCL(55O% high expression)and TCL(17% high expression),the expression of EOMES in N-NK/T-L was much higher than that in BCL(P=0.0029)and TCL(P=0.0010). MEF gene expressed in 24(96%)of 25 cases N-NK/T-L and 17 cases(68%) were high expression,and its expression in N-NK/ T-L was significantly higher than that in TCL(P=0.0050),in which only 1 case(17%)was high expression.ETS-1 expressed in all cases of N-NK/T-L and control groups.Conclusion:The expression of EOMES gene and MEF gene may function in the development of N-NK/T-L and the rumor necrosis.The general expression of ETS-1 gene indicates that it may be a fundamental common part of the program of lymphoid development and function.