The structure of the carbohydrate backbone of the LPS from Myxococcus xanthus strain DK1622 |
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| Authors: | Maclean Leann Perry Malcolm B Nossova Ludmila Kaplan Heidi Vinogradov Evgeny |
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| Institution: | Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6. |
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| Abstract: | Gram-negative rod shaped bacterium Myxococcus xanthus DK1622 produces a smooth-type LPS. The structure of the polysaccharide O-chain and the core-lipid A region of the LPS has been determined by chemical and spectroscopic methods. The O-chain was built up of disaccharide repeating units having the following structure: -->6)-alpha-D-Glcp-(1-->4)-alpha-D-GalpNAc6oMe*-(1--> with partially methylated GalNAc residue. The core region consisted of a phosphorylated hexasaccharide, containing one Kdo residue, unsubstituted at O-4, and no heptose residues. The lipid A component consisted of beta-GlcN-(1-->6)-alpha-GlcN1P disaccharide, N-acylated with 13-methyl-C14-3OH (iso-C15-3OH), C16-3OH, and 15-methyl-C16-3OH (iso-C17-3OH) acids. The lipid portion contained O-linked iso-C16 acid. |
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| Keywords: | LPS lipopolysaccharide HMBC heteronuclear multiple bond connectivity GalNAc d-galactose" target="_blank">2-acetamido-2-deoxy-d-galactose |
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