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In vitro propagation of Agave fourcroydes Lem. (Henequen) |
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| Authors: | Manuel L Robert J L Herrera F Contreras Keith N Scorer |
| Institution: | (1) Departamento de Bioquímica Vegetal, División de Estudios de Posgrado, Facultad de Química, UNAM, MEX-04510 México, D.F.;(2) Present address: División de Biología Vegetal, Centro de Investigación Científica de Yucatán, A.C., Cordemex, Apdo. Postal 87, MEX-97310, Yucatán, Mexico;(3) División de Biología Vegetal, Centro de Investigación Científica de Yucatán, A.C., Cordemex, Apdo. Postal 87, MEX-97310, Yucatán, México |
| Abstract: | In vitro plant regeneration of Agave fourcroydes Lem. (Agavaceae) is described. Results suggest that the NO3 -:NH4 + balance in the culture medium is a key factor controlling callus growth and organogenesis in rhizome cultures. Stem callus showed limited organogenic capacity, but high cytokinin concentrations induced adventitious shoot formation on stem explants. When these shoots were excised and subcultured, new callus formed at their base from which new shoots arose. The shoots from stem explants and rhizome callus formed extensive root systems in vitro and were transferred to pot culture with a 90% survival rate. |
| Keywords: | Agave fourcroydes Lem organogenesis plant tissue culture plant regeneration in vitro |
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