Heterologous hybridization of Frankia DNA to Rhizobium meliloti and Klebsiella pneumoniae nif genes |
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Authors: | Pascal Simonet Philippe Normand René Bardin |
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Institution: | Laboratoire de Biologie des Sols, U.A. CNRS 697, UniversitéClaude Bernard, Lyon I, Villeurbanne, France |
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Abstract: | A nif gene probe from Rhizobium meliloti was used to isolate a recombinant bacteriophage from a Frankia sp. ArI3 gene bank. There is a large homology between nif D and nif H genes of R. meliloti or Klebsiella pneumoniae and Frankia DNA sequences. Approximately 4.5 kb to the right of nif K, we have localized a DNA region hybridizing to a R. meliloti probe containing nif A and nif B genes. The extent of the homology was greater for nif B than for nif A. |
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Keywords: | Frankia Actinorhizal symbiosis Nif gene |
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