The effect of substrate and epidermal growth factor on human placental trophoblast cells in culture |
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Authors: | P Truman H C Ford |
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Institution: | (1) Department of Pathology, Wellington Clinical School of Medicine, Wellington Hospital, Wellington, New Zealand |
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Abstract: | Summary Attempts were made to select for trophoblast cells in cultures of mixed cell populations derived from preterm (7 to 12 wk)
or term human placentas. Epidermal growth factor added to cultures on solid or porous supports caused proliferation of epithelial-type
cells to give a confluent monolayer but did not increase the expression of differentiated function. The presence or absence
of placental basement membrane collagen as substrate made little apparent difference; however a porous basement membrane collagen
support led to increased differentiated function. Initial production of human chorionic gonadotrophin was increased and after
4 wk in culture a substantial proportion of the cells exhibited alkaline phosphatase activity. Epidermal growth factor and
a substrate of placental basement membrane collagen on a porous support favorably influence the growth and differentiation
of human trophoblast cells in culture.
This work was supported by funds from the Medical Research Council of New Zealand which also provided support for Dr. Truman
as a Postdoctoral Fellow. |
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Keywords: | placenta trophoblast cell culture substrate collagen |
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