Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii |
| |
Authors: | Ayush Kumar Courtney Dalton Herbert P Schweizer |
| |
Institution: | a Faculty of Health Sciences, University of Ontario Institute of Technology, Oshawa, Ontario, Canadab Department of Microbiology, Immunology and Pathology, Rocky Mountain Regional Center of Excellence for Biodefense and Infectious Diseases Research, IDRC at Foothills Campus, 2025 Campus Delivery, Colorado State University, Fort Collins, CO, USA |
| |
Abstract: | Acinetobacter baumannii is an emerging pathogen that causes serious infections with high mortality rates in immunocompromised individuals. Genetic manipulations in this medically-relevant pathogen are limited by the paucity of molecular tools. In this study, we show the application of the mini-Tn7-based single copy insertion system in A. baumannii. Mini-Tn7 elements are known to integrate at a naturally evolved, therefore presumably neutral location (intergenic region) downstream of the glmS gene (glucosamine-fructose-6-phosphate aminotransferase) in Gram-negative bacteria. We identified the site of insertion of mini-Tn7 in A. baumannii and demonstrated application of this useful cloning tool by inserting the gfp gene into the chromosome. Our work shows that mini-Tn7 elements are useful tools for genetic studies in this important pathogen. |
| |
Keywords: | A baumannii Mini-Tn7 Single-copy gene cloning |
本文献已被 ScienceDirect 等数据库收录! |
|