Chromosome diminution in Ascaris suum: Chromatin structure

Ascaris suum loses 56% of its nuclear DNA during chromosome diminution. Measured values of histones per nucleus are relatively constant, resulting in an approximate doubling of histone: DNA ratios during this process. Experiments were performed in an effort directed towards ascertaining the location of the increased histones. Repeat lengths of micrococcal nuclease protected pre- and post-diminution DNA were determined. Nuclear sap proteins from post-diminution nuclei were also examined in order to test the possibility of nuclear pools of free histones.