人工湿地不同植物根际微生物群落多样性比较研究

应用平板计数与PCR-DGGE技术相结合的方法,对人工湿地芦苇(Phragmites australis)、香蒲(Typha latifolia)和黑麦草(Lolium perenne)三种植物根际微生物群落多样性进行研究。结果表明,三种植物根际具有不同的微生物数量和群落结构;根际微生物数量显著高于非根际(P<0.05),两者具有不同的群落结构和优势种群;芦苇根际具有数目最多的常见物种和最大的物种均匀性,根际与根区多样性指数差别最大,根际效应R/S最大,表明芦苇能够更好的影响土壤微生物的富集和分布,提高微生物群落多样性。

Study on rhizosphere microbial community diversity of different plants grownin the constructed wetland

Rhizosphere microbial community diversity of different plants Phragmites australis, Typha latifolia, and Loliumperenne grown in constructed wetland was studied by plate count methods and PCR-DGGE. The results were as followed.Rhizosphere of different plants was different in community structure and microbial quantity. The microbial quantities inrhizosphere zone were significantly different from those in non-rhizosphere zone (P<0.05). Moreover, there were differentcommunity structure and dominant populations between rhizosphere and non-rhizosphere. Phragmites australis rhizospherehad the largest number of common species and species evenness. At the same time, it had the most significant rhizosphereeffect (R/S).The biggest different diversity index between rhizosphere and non-rhizosphere was also in P. australis, whichshowed that it could better influence the enrichment and distribution of soil microbes, and improve the microbialcommunitiy diversity.