Molecular characterization and expression analysis of a rice protein phosphatase 2C gene, OsBIPP2C1, and overexpression in transgenic tobacco conferred enhanced disease resistance and abiotic tolerance |
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| Authors: | Xuebo Hu Fengming Song Zhong Zheng |
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| Institution: | Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, P. R. China; Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, P. R. China; State Key Laboratory for Rice Biology, Zhejiang University, Hangzhou, Zhejiang 310029, P. R. China; Present address: Institute of Microbiology, Chinese Academy of Science, Beijing 100080, China |
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| Abstract: | A full-length cDNA of a rice protein phosphatase 2C gene, OsBIPP2C1 , was cloned and identified. OsBIPP2C1 is predicted to encode a 569 amino acid protein that contains phosphatase domain at its C-terminal and a relatively long N-terminal extension. Expression profiles of OsBIPP2C1 in rice seedlings upon treatments with disease resistance inducers, pathogen infection, and mechanical wounding as well as various environmental stress conditions were analyzed. Expression of OsBIPP2C1 was activated upon treatments with benzothiadiazole (BTH), salicylic acid, and hydrogen peroxide, which are signal molecules in plant disease resistance responses, and was induced during the first 48 h after inoculation with Magnaporthe grisea in BTH-treated rice seedlings. OsBIPP2C1 was also upregulated upon mechanical wounding and treatments with abscisic acid, high salt, low temperature, and drought stress. Transgenic tobacco plants overexpressing OsBIPP2C1 gene showed enhanced disease resistance against tobacco mosaic virus and Phytophthora paratisca and increased tolerance against salt and osmotic stresses. These results suggest that OsBIPP2C1 may play important roles in responses to biotic and abiotic stresses. |
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