Changes in nitrogen content and protein profiles following <Emphasis Type="Italic">in vitro</Emphasis> selection of NaCl resistant mung bean and tomato |
| |
Authors: | Email author" target="_blank">Nemat?M?HassanEmail author Mamdouh?S?Serag Fawzia?M?El-Feky |
| |
Institution: | (1) Botany Department, Faculty of Science (Damietta), Mansoura University, P.O. Box 34517 (54), New Damietta, Egypt |
| |
Abstract: | Mung bean and tomato were in vitro selected on media containing 0, 25, 50, 100 and 150 mM NaCl. Two types of media (hormone supplemented media, CB and hormone
free media, MS) were used for mung bean using cotyledon explants whereas two types of explants (cotyledons and shoot apices)
were used for tomato on MS media. Total-N, protein content, nitrite reductase (NiR) activity and protein protein profiles
were checked in selected plants and compared to original non selected ones. NaCl at low concentrations slightly increased
total-N in shoots and roots of in vitro selected mung bean and tomato whereas higher concentrations induced significant reductions. Similar increases in protein
content were detected at lower concentrations with no significant effects thereover. On the contrary, NaCl gradually inhibited
NiR activity. Similar responses of total-N, protein and NiR activity, but with greater magnitudes, were detected in original
plants. In addition, NaCl significantly reduced dry weights of shoots and roots of either in vitro selected or, in particular, original intact plants. Moreover, electrophoresis (SDS-PAGE) of protein from shoots of either
in vitro selected or intact plants showed that NaCl induced new protein bands while some others were concomitantly disappeared. The
induction of one or more of the 86.4, 79, 77.6, 77 and 71.5 kDa bands following in vitro selection and/or the disappearance of the 86 kDa band from intact plants seemed necessary for mung bean resistance. Also,
the presence of 86.2 kDa band and/or the loss of the 85.8 and 57.5 kDa bands might be included in tomato resistance. Of these
induced bands in mung bean selected on CB media, only two bands were detected in plants selected on MS media. In tomato, two
bands lost following selection from cotyledons but only one band lost following selection from shoot apices. These changes
in protein pattern therefore might serve as adaptive regulators for resistance to NaCl. |
| |
Keywords: | In vitro selection Lycopersicon esculentum nitrite reductase protein profile resistance to NaCl Vigna radiata |
本文献已被 SpringerLink 等数据库收录! |
|