Isolation, cloning and characterization of Polistes dominulus venom phospholipase A1 and its isoforms |
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Authors: | Moawad T I Hoffman D R Zalat S |
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Institution: | Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt. moawad95@hotmail.com |
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Abstract: | Polistes dominulus is known to be an allergenically important social wasp. Its venom has four major allergens (ziv. phospholipase A1, hyaluronidase, antigen 5 and a serine-protease). Amino acid sequences of its serine-protease and Antigen 5 have been published. In this paper, the partial amino acid sequence of its venom phospholipase native protein is reported. Also, we give an account to the complete nucleotide sequence of Polistes dominulus venom phospholipase A1 gene, its isoforms and their complete deduced amino acid sequences. Their similarity to the other phospholipases A1 of the family: Vespidae is discussed. |
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