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Ezrin regulates skin fibroblast size/mechanical properties and YAP-dependent proliferation
Authors:Chunji Quan  Yan Yan  Zhaoping Qin  Zhenhua Lin  Taihao Quan
Affiliation:1.Department of Pathology,Affiliated Hospital of Yanbian University Medical College,Jilin,People’s Republic of China;2.Department of Dermatology, Plastic Surgery Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China;3.Department of Dermatology,University of Michigan Medical School,Ann Arbor,USA
Abstract:Ezrin acts as a dynamic linkage between plasma membrane and cytoskeleton, and thus involved in many fundamental cellular functions. Yet, its potential role in human skin is virtually unknown. Here we investigate the role of Ezrin in primary skin fibroblasts, the major cells responsible extracellular matrix (ECM) production. We report that Ezrin play an important role in the maintenance of skin fibroblast size/mechanical properties and proliferation. siRNA-mediated Ezrin knockdown decreased fibroblast size and mechanical properties, and thus impaired the nuclear translocation of YAP, a protein commonly response to cell size and mechanical force. Functionally, depletion of Ezrin significantly inhibited YAP target gene expression and fibroblast proliferation. Conversely, restoration of YAP nuclear translocation by overexpression of constitutively active YAP reversed YAP target genes expression and rescued proliferation in Ezrin knockdown cells. These data reveal a novel role for Ezrin in maintenance of fibroblast size/mechanical force and regulating YAP-mediated proliferation.
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