Preparation and characterization of epoxy-functionalized magnetic chitosan beads: laccase immobilized for degradation of reactive dyes |
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| Authors: | Gulay Bayramoglu Meltem Yilmaz M Yakup Arica |
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| Institution: | (1) Biochemical Processing and Biomaterial Research Laboratory, Department of Chemistry and Biology, Faculty of Arts and Sciences, Gazi University, Teknikokullar, 06500 Ankara, Turkey;(2) Department of Environmental Sciences, Institute of Science and Technology, Gazi University, 06570 Ankara, Turkey |
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| Abstract: | Cross-linked magnetic chitosan beads were prepared by phase-inversion technique in the presence of epichlorohydrin under alkaline
condition, and used for covalent immobilization of laccase. The activity of the immobilized laccase on the magnetic chitosan
was about 260 U (g/dry beads) with an enzyme loading of about 16.33 ± 0.39 mg (g/dry beads) mg/g]. Kinetic parameters, V
max and K
m values were determined as 21.7 U/mg protein and 9.4 μM for free enzyme, and 15.6 U/mg protein and 19.7 μM for the immobilized
laccase, respectively. The operational and thermal stabilities of the immobilized laccase were improved compared to free counterpart.
The immobilized laccase was operated in a batch reactor for the decolorization of reactive dyes from aqueous solution. The
laccase immobilized on magnetic chitosan beads was very effective for removal of textile dyes from aqueous solution which
creates an important environmental problem in the discharged textile dying solutions. |
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