Quantification of melatonin in human saliva by liquid chromatography-tandem mass spectrometry using stable isotope dilution |
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Authors: | Eriksson Kåre Ostin Anders Levin Jan-Olof |
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Institution: | 1. Department of Occupational and Environmental Medicine, University Hospital of Northern Sweden, SE-901 85 Umeå, Sweden;2. National Institute for Working Life, SE-907 13 Umeå, Sweden |
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Abstract: | A method for the determination of melatonin in human saliva has been developed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS). Saliva was collected in plastic tubes. 7-D-Melatonin was added as internal standard and the samples were cleaned and concentrated by solid-phase extraction. The limit of detection was 1.05 pg x ml(-1) and the limit of quantification was 3.0 pg x ml(-1). The accuracy of the method was +/-14% at 5.60 pg x ml(-1) and +/-9% at 19.6 pg x ml(-1). The precision was +/-13% at 6.18 pg x ml(-1) and +/-11% at 31.2 pg x ml(-1), respectively. Our HPLC-MS-MS method shows a high sensitivity and specificity for melatonin and more reliable results compared with a radioimmunoassay. The chromatographic method has been used to determine the circadian rhythm of melatonin among three nurses working the night shift and a patient suffering from an inability to fall asleep at night. |
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Keywords: | Melatonin |
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