In vitro conversion of testosterone-4-14C to androgens of the 5alpha-androstane series by a normal human ovary |
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| Authors: | O W Smith P Ofner R L Vena |
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| Institution: | Fearing Research Laboratory, Boston Hospital for Women, Parkway Division, Brookline, Mass. 02146, and Steroid Biochemistry Laboratory, Lemuel Shattuck Hospital, Department of Urology, Tufts University School of Medicine and Departments of Pharmacology, Harvard School of Dental Medicine and Harvard Medical School, Boston, Mass.U.S.A. |
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| Abstract: | In a previous communication (1) the identification of Δ4 -3-oxo-steroids and estrogens as metabolites of testosterone-4-14C incubated with normal post-ovulatory human ovaries was reported. Thin-layer chromatography of the extracts of those ovaries which contained no corpus luteum yielded zones of radioactivity which were not associated with any of these products. Detailed investigation of these zones from the extract of one of these glands resulted in identification of the following radiometabolites of the 5α-androstane series: 5α-androstane-3,17-dione, androsterone, 3β-hydroxy-5α-androstan-17-one, 17β-hydroxy-5α-androstan-3-one, 5α-androstane-3ga, 17β-diol and 5α-androstane-3β, 17β-diol. The capacity of a normal human ovary to produce these 5α-reduced androgens, especially the potent 17β-hydroxy-steroids, suggests a regulatory role of these compounds in ovarian function. |
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