Differences in reactivity of confluent and nonconfluent cultures of human endothelial cells toward thrombin-stimulated platelets or heparinized salt solution |
| |
Authors: | Steinar Solberg Tor Larsen Leif Jørgensen |
| |
Institution: | 1. Section of Morphology II, Institute of Medical Biology, University of Troms?, Norway
|
| |
Abstract: | Summary This study examined whether nonconfluent endothelial cell cultures reacted differently than confluent ones toward thrombin-stimulated
platelets or a heparinized salt solution. The adherence to the endothelial cell cultures of51Cr-labeled human platelets stimulated at different thrombin concentrations was studied. There was significantly higher adherence
of stimulated platelets to nonconfluent cultures compared with confluent ones. This was confirmed by scanning electron microscopy,
which also revealed a tendency for the platelets to adhere at the cell periphery. Electron microscopy also showed that thrombin-stimulated
platelets induced endothelial cell contraction. Part of the peripheral endothelial cell surface toward the bottom of the culture
dish was inverted, facing the lumen of the dish. This phenomenon was particularly seen in nonconfluent cultures. When51Cr-labeled endothelial cultures were incubated with a mildly injurious fluid as heparinized sodium acetate and 20% serum,
at 20° C for 30 min, the nonconfluent cultures showed significantly more cell detachment and release of51Cr than the confluent ones. We conclude that under the conditions of the present experiments there are differences in the
reactivity of confluent and nonconfluent endothelial cell cultures. These differences probably reflect biological dissimilarities.
In experiments where properties of cultured endothelium are studied, care should be taken that the degree of confluency is
standardized. |
| |
Keywords: | cell density endothelial cell cultures human platelets thrombin platelet adherence heparinized salt solution endothelial cell injury |
本文献已被 SpringerLink 等数据库收录! |