The Intracellular Location of Catechol Oxidase: in the Olive Fruit |
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| Authors: | SHOMER I; BEN-SHALOM N; HAREL E; MAYER A M |
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| Institution: | Division of Food Technology, Agricultural Research Organization, The Volcani Center Bet Dagan, Israel
Botany Department, The Hebrew University of Jerusalem Jerusalem, Israel |
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| Abstract: | Catechol oxidase in young olive fruits was located on the innerface of chloroplast thylakoids and in the mitochondria. Thiswas demonstrated by observing the oxidation products of 3,4-dihydroxyphenyl-alanine(DOPA) with the electron microscope in fruit segments prefixedwith glutaraldehyde. Diethyl dithiocarbamate (DIECA), an inhibitorof the enzyme, prevented the accumulation of oxidation productsof DOPA. Olea europaea L, olive fruits, catechol oxidase, ultrastructure |
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