纤维素酶-超声波协同提取黑木耳黑色素工艺及其抗氧化活性分析

黑色素是黑木耳的主要活性成分之一,在黑木耳的药理活性上发挥着重要作用。为了提高黑木耳黑色素的提取得率,实验采用正交法和响应面法对纤维素酶-超声波协同提取黑木耳黑色素的提取工艺进行了优化,并对最优条件下提取的黑木耳黑色素体外抗氧化活性进行了分析。实验结果表明,纤维素酶-超声波协同提取黑木耳黑色素的最优条件为:酶添加量12mg,酶解温度40℃,酶解pH 5.0,酶解时间120min,NaOH浓度1.27mol/L,料液比1:40,超声功率300W,超声时间52min,超声温度60℃。在最优条件下,黑木耳黑色素提取得率可达到10.48%,相比于实验设置的未添加纤维素酶的超声波组黑色素提取得率提高了12.93%。抗氧化结果表明,采用纤维素酶-超声波协同提取的黑色素相比于未加纤维素酶提取的黑色素清除ABTS、DPPH和羟基自由基的能力更强。研究结果为黑木耳黑色素的高效提取及其产品的应用开发奠定了基础。

Cellulase and ultrasonic wave synergistic extraction technology of melanin from Auricularia heimuer and analysis of antioxidant activity of the melanin product

Melanin is one of the main active components of Auricularia heimuer and plays a significant role in pharmacological activities of Auricularia heimuer. Orthogonal and response surface methods were used to optimize the Auricularia heimuer melanin extraction process by cellulase and ultrasonic wave synergistic technology in order to improve the extraction yield of melanin. The antioxidant activity of the melanin product was analysed. Experimental results show that the optimum conditions for the synergistic extraction of melanin include enzyme additive quantity of 12mg, enzymolysis temperature of 40°C, enzymolysis pH of 5.0, enzymolysis duration of 120min, NaOH concentration of 1.27mol/L, solid-liquid ratio of 1:40, ultrasonic power of 300W, ultrasonic duration of 52min, and ultrasonic temperature of 60°C. Under such conditions, the extraction yielding rate of Auricularia heimuer melanin could reach 10.48%, 12.93% higher as compared with experimental group treated with ultrasound without addition of cellulase. The melanin product obtained from the cellulose-ultrasound synergistic process had more scavenging effects on ABTS, DPPH and hydroxyl radical as compared with that obtained from the extraction using only cellulose without ultrasonic treatment.