黄淮海地区玉蜀黍黑粉菌群体遗传结构分析

利用17个多态性ISSR引物对2017年在我国黄淮海地区采集的41株玉蜀黍黑粉菌Ustilago maydis样本进行了种群遗传结构分析。基于UPGMA法和贝叶斯模型对玉蜀黍黑粉菌群体遗传结构分析显示,采集的样本可以分为两大群,且种群的划分表现出了与来源地理纬度的一致性。因此按照各样本所属地理纬度将样本分为北纬33°-34°、北纬35°-36°、北纬37°-38° 3大种群。结果表明,分离自北纬33°-34°地区的玉蜀黍黑粉菌遗传多样性最丰富,Nei’s基因多样性（H）为0.2216,Shannon指数（I）为0.3300。分子方差分析（AMOVA）显示,变异中来自种群内部的变异为86%,种群间变异14%（φpt=0.141）。

Population genetic structure of Ustilago maydis collected from Huang-Huai-Hai region

To determine the population genetic structure of Ustilago maydis, 41 isolates collected from Huang-Huai-Hai region in 2017 were analyzed by using 17 polymorphic ISSR primers. The unweighted pair group method with arithmetic mean (UPGMA) and the Bayesian analysis were used to analyse the population genetic structure of the collected isolates. It was indicated that the isolates could be divided into two geographical groups, and the groups showed clear relationship with the geographical origin of the isolates. Based on demarcation of geographical latitude, the isolates were clustered into 3 groups, 33°-34° N group, 35°-36° N group and 37°-38° N group. The genetic diversity of the 33°-34° N group was the highest. Nei’s gene diversity (H) and Shannon index (I) was 0.2216 and 0.3300, respectively. An estimation of the genetic diversity using analysis of molecular variance (AMOVA) revealed that the highest variance mainly formed in intrapopulation (86%), and subordinately interpopulation (14%) (φpt=0.141).