不同训练方法对大鼠骨折后脊髓功能恢复的影响

摘要 目的：分析不同训练方法对大鼠骨折后脊髓功能恢复的影响。方法：随机选取40只Sprague Dawley (SD)大鼠，建立骨折合并脊髓损伤模型，另取10只大鼠作为正常组。将建模成功的SD大鼠随机分为模型组、减重平板训练组、游泳训练组和转笼训练组。分别于损伤前和损伤后对大鼠的运动功能进行评测；术后35 d对大鼠的运动诱发电位（(motor evoked potentials, MEP）、脑源性神经生长因子（brain-derived neuotrophyic factor, BDNF）、神经元特异性烯醇化酶（neuron-specific enolase, NSE）和脊髓组织Cleaved Caspase-3表达量、肌纤维横截面积和直径进行评测。结果：损伤后，手术组大鼠运动功能评分均降低；经不同方式训练后，大鼠的运动功能评分均上升，14 d～35 d数据差异有统计学意义（P＜0.05）。术后35 d，与模型组相比，训练组大鼠运动诱发电位潜伏期均缩短，波幅、BDNF和NSE表达量、肌纤维横截面积和直径均增大，Cleaved Caspase-3表达量均降低，14 d～35 d数据差异有统计学意义（P＜0.05）。其中，减重平板训练组各指标检测结果最优。结论：三种训练方式均可促进大鼠骨折后脊髓功能恢复，其中减重平板训练组恢复效果＞游泳训练组＞转笼训练组。

Effects of Different Training Methods on the Recovery of Spinal Cord Function after Fracture in Rats

ABSTRACT Objective: To analyze the Effects of different training methods on the recovery of spinal cord function after fracture in rats. Methods: Forty SD rats were randomly selected to establish the model of fracture combined with spinal cord injury, and another 10 rats were selected as the normal group. SD rats were randomly divided into model group, weight-loss training group, swimming training group and rotating cage training group. The motor function of rats was evaluated before and after injury. The motor evoked potential (MEP), brain-derived nerve growth factor (BDNF), neuron specific enolase (NSE) and cleaved caspase-3 expression in spinal cord tissue, cross-sectional area and diameter of muscle fibers were measured 35 days after operation. Results: After the injury, the motor function scores of the rats in the operation group decreased. After training in different ways, the score of motor function of rats increased, and the difference was statistically significant (P<0.05). On the 35th day after operation, compared with the model group, the latency of motor evoked potential in the training group was shortened, the amplitude, the expression of BDNF and NSE, the cross-sectional area and diameter of muscle fiber were increased, and the expression of cleaved caspase-3 was decreased, and the difference between the 14 d and 35 d was statistically significant (P<0.05). Among them, the test results of each index of the weight-loss plate training group are the best. Conclusion: Three training methods can promote the recovery of spinal cord function after fracture in rats, among which the recovery effect of weight-loss plate training group > swimming training group > rotating cage training group.