Identification of novel ribonucleo-protein complexes from the brain-specific snoRNA MBII-52 |
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Authors: | Yuuichi Soeno Yuji Taya Taras Stasyk Lukas A Huber Takaaki Aoba Alexander Hüttenhofer |
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Institution: | 1.Department of Pathology, School of Life Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, 102-8159 Tokyo, Japan;2.Division of Genomics and RNomics, Biocenter, Innsbruck Medical University, A-6020 Innsbruck, Austria;3.Division of Cell Biology, Biocenter, Innsbruck Medical University, A-6020 Innsbruck, Austria |
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Abstract: | Small nucleolar RNAs (snoRNAs) guide nucleotide modifications within ribosomal RNAs or spliceosomal RNAs by base-pairing to complementary regions within their RNA targets. The brain-specific snoRNA MBII-52 lacks such a complementarity to rRNAs or snRNAs, but instead has been reported to target the serotonin receptor 2C pre-mRNA, thereby regulating pre-mRNA editing and/or alternative splicing. To understand how the MBII-52 snoRNA might be involved in these regulatory processes, we isolated the MBII-52 snoRNP from total mouse brain by an antisense RNA affinity purification approach. Surprisingly, by mass spectrometry we identified 17 novel candidates for MBII-52 snoRNA binding proteins, which previously had not been reported to be associated with canonical snoRNAs. Among these, Nucleolin and ELAVL1 proteins were confirmed to independently and directly interact with the MBII-52 snoRNA by coimmunoprecipitation. Our findings suggest that the MBII-52 snoRNA assembles into novel RNA-protein complexes, distinct from canonical snoRNPs. |
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Keywords: | noncoding RNA small nucleolar RNA MBII-52 Nucleolin ELAVL1 |
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