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Efficient mRNA detection from human archival paraffin-embedded tissue: An update |
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| Authors: | Romana Illig Helga Fritsch Christoph Schwarzer |
| Affiliation: | 1.Department of Anatomy, Histology, and Embryology, Division of Clinical and Functional Anatomy, Innsbruck Medical University, 6020 Innsbruck, Austria;2.Department of Pharmacology, Innsbruck Medical University, 6020 Innsbruck, Austria |
| Abstract: | We recently published an in situ hybridization protocol for archival tissue using a commercial hybridization buffer. This buffer is no more available. Therefore, we have developed an improved protocol with a defined hybridization buffer. |
| Keywords: | target retrieval human ontogeny chromogenic mRNA detection defined hybridization buffer |