Difference in the expression pattern of dystrophin on the surface membrane between the skeletal and cardiac muscles of mdx carrier mice |
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Authors: | H Tanaka K Ikeya E Ozawa |
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Institution: | (1) Division of Cell Biology, National Institute of Neuroscience, NCNP, Kodaira, 187 Tokyo, Japan |
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Abstract: | Summary We examined the expression of dystrophin by immunohistochemical and immunoblot analyses in the skeletal and cardiac muscles
of Xmdx/X+ heterozygous mice, which were obtained by mating male mdx mice (Xmdx/Y) with female wild type mice (X+/X+). Dystrophin was expressed on the surface membrane in both muscles, but the mode of expression was different between the
two muscles. In cardiac muscle, dystrophin positive and negative cells were present in roughly equal numbers intermingled
in a mosaic pattern; this was considered to reflect the random inactivation of X-chromosomes in early development. In skeletal
muscle, most of the surface membrane was dystrophin positive. There were little signs of fiber necrosis or regeneration, and
serum creatine kinase levels were normal. We are at present of opinion that the predominance of dystrophin-positive area in
skeletal muscle is due to intracellular diffusion of dystrophin.
On leave from The Department of Pediatrics, Tokyo Women's Medical College |
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