A novel extranuclear mutant of Neurospora with a temperature-sensitive defect in mitochondrial protein synthesis and mitochondrial ATPase |
| |
Authors: | Richard A Collins Helmut Bertrand Robert J LaPolla and Alan M Lambowitz |
| |
Institution: | (1) Department of Biology, University of Regina, S45 OA2 Regina, Saskatchewan, Canada;(2) The Edward A. Doisy Department of Biochemistry, St. Louis University School of Medicine, 63104 St. Louis, Missouri, USA |
| |
Abstract: | Summary C93] is a novel, extranuclear mutant of Neurospora crassa which has a normal mitochondrial phenotype when grown at 25°, but which is deficient in cytochromes b and aa
3 when grown at 37° (Pittenger and West 1979). In the present work, the phenotype of C93] was characterized in greater detail. When C93] is grown at 37°, the rate of mitochondrial protein synthesis is decreased to approximately 25% that of wild type; the ratio of mitochondrial small to large ribosomal subunits is decreased to 1:4 and mitochondrial small subunits are deficient in the mitochondrially-synthesized protein, S-5. The mitochondrial ribosome assembly defects in 37°-grown C93] resemble those in chloramphenicol-treated wild-type cells and could merely be a consequence of the decreased rates of mitochondrial protein synthesis. Analysis of mitochondrial translation products by SDS gel electrophoresis suggests that 37°-grown C93] is grossly deficient in the 19,000 Mr subunit of the oligomycin-sensitive ATPase relative to other mitochondrially-synthesized proteins. The ATPase defect was not found in other extranuclear or nuclear mutants deficient in mitochondrial protein synthesis. These data and additional evidence suggest that the primary defect in C93] may be in the assembly of the ATPase complex. The possible connection between the ATPase defect and the deficiency of mitochondrial protein synthesis is discussed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|