细弱绒泡菌的生活史

利用燕麦-琼脂培养、基物培养及扫描电镜技术研究了细弱绒泡菌的个体发育过程,在燕麦琼脂培养基上完成了从孢子到孢子的生活史。结果表明,细弱绒泡菌生活史包括单核的黏变形体或游动胞、多核的营养体原质团以及孢子形成阶段。孢子球形,表面具细小疣点。孢子萌发为裂式,释放1黏变形体。黏变形体行变形运动,在有水的条件下,可转变为游动胞。成熟原质团橘黄色。原质团类型为显型,具有扇形网络状菌脉。成熟原质团可形成多个孢囊。琼脂培养基上获得的细弱绒泡菌孢子与野生型相似,并具有可育性。

Life cycle of Physarum tenerum

Oat-agar culture method,moist chamber culture method and scanning electron microscopy were used to study the process of the individual development of Physarum tenerum.The life cycle of spore-to-spore were completed in agar culture in Petri dish.The result shows that the life cycle of Physarum tenerum comprises a unicellular amoeboid or swarm cell stage,a multinucleate plasmodium stage and a sporulation stage.The spore of Physarum tenerum is globose and the spore surface is minutely spiny.The spore germinates by means of a crack open and releases a single myxamoeba.The myxamoeba can move by amoeboid motion.When free water is available,myxamoebae can transform into swarm cells.The mature plasmodium is orange.The plasmodial type is the phaneroplasmodium which appears as a fan-shaped network of veins.The mature plasmodium can form many sprangia.Spores developed on agar are fertile and resemble those on natural substrate.