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Genetic transformation ofAjuga multiflora bunge withAgrobacterium rhizogenes and 20-hydroxyecdysone production in hairy roots |
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| Authors: | Ok Tae Kim M. Manickavasagm Young Jun Kim Mei Ran Jin Kwang Soo Kim Nak Sul Seong Baik Hwang |
| Affiliation: | (1) Department of Biology and Institute of Plant Resources, Chonnam National University, 500-757 Gwangju, Korea;(2) Mokpo Experiment Station, National Institute of Crop Science, RDA, 534-833 Muan, Korea;(3) National Institute of Crop Science, RDA, 441-857 Suwon, Korea;(4) Department of Biotechnology, Bharathidasan University Tiruchirapalli, 620-024 Tamil Nadu, India |
| Abstract: | An efficient transformation system forAjuga multiflora Bunge was established by usingAgrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 20-hydroxyecdysone (20-HE). PCR amplification of rooting locus (rol) genes revealed that the left hand-transferred DNA of the root-inducing plasmid was inserted into the genome of our transformedAjuga hairy roots. This integration was further confirmed by DNA-DNA hybridization. The 20-HE content in hairy roots was 10 times higher than that measured in the wild type. |
| Keywords: | Ajuga multiflora hairy roots 20-hydroxyecdysone Ri-plasmid rol genes |
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