电磁脉冲对C57BL/6J 胸腺的作用

目的:观察电磁脉冲Electromagnetic Pulse(EMP)对C57BL/6J小鼠胸腺的影响。方法:50只C57BL/6J小鼠按体重区组随机化分为对照组和辐照组,每组25只。EMP每天照射400次,连续照射7天,照后1d(天)、3d、7d、14d、28d共5个时间点杀取胸腺。2只辐照组和2只对照组杀取的胸腺做HE染色,观察其病理改变;3只辐照与3只对照组的小鼠,杀后称取小鼠的体重和胸腺的重量,计算胸腺指数;然后提取T淋巴细胞进行计数;同时取小鼠外周血检测其中的IL-4的水平。结果:照后1d胸腺的切片没有明显改变。7天后,胸腺开始有出血,结构不清;胸腺指数呈现递减的趋势,但辐照组与对照组没有的差异没有统计学意义;T淋巴细胞数的变化也呈现先减后增的趋势,在第1d、14d、28d辐照组与对照组的差异没有统计学意义,在第3d和7d辐照组的细胞数小于对照组的细胞数(P<0.05);辐照组与对照组的外周血IL-4水平的差异也没有统计学意义。结论:电磁脉冲对雄性Balb/c小鼠胸腺结构造成一定的损伤,但胸腺指数改变不显著,T淋巴细胞数量增加。表明EMP对胸腺有一定的作用,但是胸腺不是EMP作用的敏感器官。

Effects of Electromagnetic Pulse on the T Lymphocyte in the Thymus of Mice

Objective: To investigate the effect of electromanetic pulse on T lymphocyte of thymus in the C57BL/6J mouse.Methods: 50 male Balb/c mice,weighing(18～22)g,were sham or whole-body exposed to EMP at 200kV/m for 400 pulses per day,which lasted for one week.The thymus from the mice on the 1st d(day),3rd d,7th d,14th d and 28th d after exposion wad used.The thymus from two mice of the exposive group and two mice of the sham group were cut and stained with hematoxylin-eosin,then the pathological changes was detected by optical microscope;The weight of the body and thymus of 3 mice of the exposive group and 3 mice of the sham group were weighted for the thymus index;The lymphocyte was extracted from the thymus;Then the blood serum was collect to check out the content of the IL-4 in the peripheral blood.Results: There was no pathological changes on the first and third days after exposion,however,there was hemorrhage,the structure of thymus changes from the 7th day;The thymus index showed the trend of decline,but there was no difference between the sham group and the exposive group;The number of T lymphocyte between the sham group and the exposive group on the 1st,14th and 28th day had no significant deviation,but the difference on the 3rd and 7th day had satistical meaning(P<0.05);The difference of the content of IL-4 in the peripheral blood between the sham group and the exposive group had no satistical meaning.Conclusions: The structure of the thymus was destroyed,the thymus index was in the declining trend,the number of T lymphocyte in the thymus declined,and the content of IL-4 had no difference.EMP had some effects on T lymphocyte in the thymus,but it is not the sensitive target of EMP on the C57BL/6J mouse.