锰对PC12 细胞的增殖抑制与凋亡研究

目的:研究锰作用下PC12细胞的增殖抑制作用与凋亡相关的形态学、生化指标改变。方法:用200,400,600,800μmol/LMnCl2的培养液,分别作用对数生长期PC12细胞1,2,3,4d后,用MTT筛选锰的细胞毒性剂量;透射电镜观察细胞形态学变化;琼脂糖凝胶电泳检测MnCl2对PC12细胞基因组DNA的影响。结果:MTT实验显示200-800μmol/L MnCl2作用4天对PC12有显著的抑制作用,呈剂量和时间依赖趋势,600μmol/L MnCl2作用4d对PC12的抑制率可达50%以上。600μmol/L MnCl2作用4d电镜可见细胞凋亡,同样条件下细胞DNA碎片化。结论:PC12细胞在锰作用下发生增殖抑制,原因是锰诱导PC12细胞凋亡。

Oxidative Stress Mechanism of Manganese-treated PC12 Cell Line

Objective: To investigate the apoptosis effect of Mn on the PC 12 cell line by detecting the cell morphology and biochemical changes.Methods: PC12 cells in logarithm period incubated in medim with 200,400,600,800μmol/L manganese(MnCl2) for 1day,2 days,3 days,4days respectively;The cell viability was detected by MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrasoliumBromide];Morphological changes of PC12 cells was investigated by transmisssion electron microscope;Agarose gel electrophoresis was used to detect the genomic DNA of Mn-treated PC12 transmisssion electron microscope as well as biochemical hallmark of DNA fragments.Results: The results of MTT revealed that manganese of different Concentrations(MnCl2200,400,600,800μmol/L) could suppress the proliferation of PC12 cells in dose and time-dependent manner.The cell inhibited ratio at the fourth day in 600μmol/L MnCl2 culture medium approached 50% or more.In the same condition apoptosis was observed in cells.Conclusion: Mn has generated apoptosis which induced proliferation arrested of PC12 cells.