芪丹通脉片促进骨髓间充质干细胞向缺血心肌趋化迁移

目的:研究芪丹通脉片对骨髓间充质干细胞向缺血心肌趋化迁移的作用。方法:SD大鼠,随机分为单纯移植组,益气组,活血组。芪丹通脉片组,各组大鼠灌服中药14天后,采用冠状动脉左前降支结扎法建立心肌梗死模型。经尾静脉注入DIO标记的骨髓间充质干细胞,3天后取缺血部位的心肌,应用流式细胞仪分析计算出每克心肌所含有的DIO阳性细胞数;行冰冻切片,采用荧光显微镜观察DIO阳性细胞;4周后采用多导生理记录仪记录大鼠平均动脉压(MAP)、左心室收缩峰压(LVPSP)、左心室内压最大上升变化速率(+LVdp/dtmax)和最大下降速率(-LVdp/dtmax)。结果:芪丹通脉片组较益气组、活血组、单纯移植组,每克心肌所含有的DIO阳性细胞数增多,其差异有统计学意义;冰冻切片镜下观察,芪丹通脉片组阳性细胞数较益气组、活血组、单纯移植组增多,其差异有统计学意义;心功能检测示:芪丹通脉片组MAP、LVPSP、+LVdp/dtmax、-LVdp/dtmax较益气组、活血组、单纯移植组改善明显,差异有统计学意义。结论:芪丹通脉片具有促进骨髓间充质干细胞向缺血心肌趋化迁移的作用,益气与活血中药配伍应用优于单纯益气药或活血药。

The Promotion of Qidantongmai Tablet on Bone Marrow Mesenchymal Stem Cells Migrating to Ischemic Myocardium

Objective:To investigate the effective of Qidantongmai tablet on Bone marrow Mesenchymal stem cells migrating to ischemic myocardium.Methods: SD rats were divided into four groups randomly,the transplantation only group,yiqi group,huoxue group,and Qidantongmai tablet group.SD rats of each group were administered with traditional Chinese medicine once everyday,respectively.It was lasted for 14 days.The model of myocardial infarction was built by ligation of the left anterior descending artery.The DIO-labeling MSCs were injected to animals through tail vein.After three days,ischemic myocardium were collected,Flow Cytometry was used to analyze the numbers of DIO-labeling cells in ischemic myocardium of every unit among each group;To observe DIO-labeling cells in myocardium frozen sections through fluorescence microscope.After 4 weeks,myocardial function of SD rats in every group were recorded by multichannel physiologic recorder,including mean arterial pressure(MAP),left ventricular peak systolic pressure(LVPSP),+LVdp/dtmax,-LVdp/dtmax.Results: The numbers of MSCs were significantly greater in Qidantongmai tablet group than that in other groups.Significant improvements of myocardial function,including MAP,LVPSP,+LVdp/dtmax,-LVdp/dtmax,in Qidantongmai tablet group prior to that in other groups.Conclusions: Qidantongmai tablet promotes Bone marrow Mesenchymal stem cells to migrate to ischemic myocardium.