镁补充对2 型糖尿病大鼠糖脂代谢的影响

目的:研究镁补充对2型糖尿病大鼠糖脂代谢的影响。方法:将用高脂饮食联合链脲佐菌素(STZ)方法诱发的2型糖尿病大鼠随机分为四个组,糖尿病对照组喂饲高脂饲料,高、中、低剂量组在高脂饲料中分别加入2000、1000、200 mg/kg的镁(以镁离子计)。自由饮食喂养四周,处死动物。用放射免疫法测血清胰岛素(Ins)水平、用葡萄糖氧化酶法测空腹血浆葡萄糖(fasting plasmaglucose,FPG),并计算胰岛素敏感指数(insulin sensitivity index,ISI)。比色法检测糖化血红蛋白(glycosylated hemoglobin,HbA1c)。用全自动生化分析仪测高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDLC)、甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)。结果:高剂量组的空腹血糖、空腹血清、糖化血红蛋白、甘油三酯、总胆固醇水平均较糖尿病对照组显著性降低(P<0.05),而高密度脂蛋白胆固醇、胰岛素敏感指数较糖尿病对照组显著性升高(P<0.05)。结论:镁补充可以提高2型糖尿病大鼠胰岛素敏感性,改善糖尿病大鼠的糖脂代谢情况。

The Influence of Magnesium Supplementation on Glucose and LipidMetabolism of Rats with Type 2 Diabetes Mellitus

Objective: To investigate the effect of magnesium supplementation on glucose and lipid metabolism of rats with type 2 diabetes mellitus.Method: Rats with type 2 diabetes mellitus were induced with high fat diet and intraperitoneal injection with small dose of STZ,then they were divided into 4 groups randomly: high,medium,low magnesium supplementation groups and diabetes mellitus control group.Diabetes mellitus control group was feed with high-fat feed only.The dosage added in high-fat feed of the high,medium,low magnesium supplementation groups are 2000,1000,200 mg/kg(measured by Mg2+).All the rats ate and drunk freely.All rats were killed after four weeks.The serum insulin(Ins) level were tested with radiation immune method,fasting plasma glucose(FPG)were mea-sured with glucose oxidase method,and calculation the insulin sensitivity index(ISI).Glycosylated hemoglobin(HbA1c) was measured by chromatometry.High-density lipoprotein cholesterol(HDLC),triglyceride(TG),total cholesterol(TC) were measured by automatic biochemical analyzer.Results: FPG,Ins,TG,TC levels of high dose group was significantly lower compared with that in the diabetic control group(P<0.05),while HDLC,ISI increased significantly compared with that in the diabetic control group(P<0.05).Conclusion: Magnesium supplement can improve insulin sensitivity,improve glucose and lipid metabolism in type 2 diabetic rats.