支气管哮喘病儿外周血树突状细胞功能变化

目的:观察支气管哮喘病儿外周血单个核细胞(PBMC)来源树突状细胞(DC)功能变化。方法:以18例健康儿童为对照,选择16例支气管哮喘发作期病儿为研究对象,分离PBMC并经rhGM-CSF诱生成熟DC。采用流式细胞仪(FACS)检测DC表面共刺激分子CD80(B7-1)、CD86(B7-2)和CD83的表达率;ELISA法检测培养上清液中IL-10和IL-12的变化。结果:①哮喘组DC表面CD86的表达率明显高于健康对照组(t=2.27,P<0.05),CD80、CD83的表达率与健康对照组比较均无显著性差异(t=1.17,1.34;P>0.05)。②哮喘组DC分泌IL-10、IL-12水平均明显低于健康对照组(t’=3.31,3.39;P<0.01)。③哮喘组DC分泌IL-10与IL-12成正相关(r=0.740,P<0.01),而健康对照组IL-10与IL-12无相关性(r=0.232,P>0.05)。结论:支气管哮喘病儿DC存在功能缺陷,主要表现在CD86表达升高、IL-10、IL-12分泌减少。

Function Changes of Dendritic Cells in Peripheral Blood of Children with Bronchial Asthma

Objective: To investigate the function changes dendritic cells(DCs) from peripheral blood mononuclear cells(PBMC) in bronchial asthmatic children.Methods: PBMC-derived immature DCs(iDCs) were obtained from peripherial blood samples in 16 asthmatic patients and in 18 healthy children.iDCs were stimulated with rhTNF-α as mature DCs.The expression of the co-stimulatory molecules CD80(B7-1),CD86(B7-2) and CD83 were detected by fluorescent activated cell sorter and the levels of IL-10 and IL-12 in the supernatant were determined by enzyme-linked immunosobent assay.Results: ①The expression of CD86 on DCs was significantly higher than that of healthy control group(t=2.27,P < 0.05).There was no significant difference in the expression of CD80,CD83 between the two groups(t=1.17,1.34;P > 0.05).②The level of IL-10 and IL-12 produced from DCs of asthmatic group were significantly lower than that of healthy control group(t’=3.31,3.39;P < 0.01).③IL-10 had postively relationship with IL-12 in asthma group(r=0.740,P<0.01),while there was no correlation in healthy control group(r=0.232,P > 0.05).Conclusion: DCs dysfunction existed in asthmatic children.It mainly manifested in the increase of the expression of CD86 and the decrease of the secretion of IL-10 and IL-12.