显微摄像条件对细胞形态测试结果的影响

目的:研究显微摄像条件对细胞形态测试结果的影响情况。方法:以苏木精-伊红(HE)染色的胃壁细胞为研究对象,分别在不同光亮度、对比度、饱和度和锐度的成像条件下进行显微摄像,采用图像分析软件(Image-Pro Plus 6.0)测试胃壁细胞的色度学和几何形态学参数,并对测试结果进行分析比较。结果:不同光亮度、对比度、饱和度组的胃壁细胞红、绿、蓝三基色差异均有统计学意义(P<0.05),其中红、绿、蓝基色值在高光亮度和高对比度组最高,同时红基色值在高饱和组最高,而绿、蓝基色值在高饱和组最低(P<0.05);不同锐度的胃壁细胞红、绿、蓝三基色差异没有统计学意义(P>0.05)。不同光亮度、对比度、饱和度和锐度的胃壁细胞的面积、周长、平均直径差异均没有统计学意义(P>0.05)。结论:光亮度、对比度和饱和度对细胞色度学参数影响明显,而对几何形态学参数无明显影响。

Effect of Microscopic Photography Conditions on Test Results of Cell Morphology

Objective: The aim of this study is to study effect of microscopic photography conditions on test results of cell morphology.Methods: The gastric parietal cells by hematoxylin-eosin(HE) staining were taken as the research object.The microscopic photography was taken in different imaging condition,including brightness,contrast,saturation and sharpness.The parameters of colorimetry and geometric morphology in gastric parietal cells were tested by image analysis software(Image-Pro Plus 6.0),and comparison and analysis was performed among test results.Results: There were statistical significances in red,green,blue three primary colors of gastric parietal cells among different brightness,contrast,saturation group(P<0.05).The high brightness and contrast group had highest values of red,green,blue three primary colors,and the high saturation group had highest values of red primary colors and lowest green,blue primary colors(P<0.05).There was no statistical significance in red,green,blue three primary colors of gastric parietal cells among sharpness group(P>0.05).There were no statistical significances in area,perimeter,average diameter of gastric parietal cells among groups(P>0.05).Conclusions: The brightness,contrast and saturation has significant influence on cell colorimetry parameters,but has not obvious effect on geometric morphology parameters.