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Determining the frequency of heteropteran predation on sweetpotato whitefly and pink bollworm using multiple ELISAs
Authors:James R. Hagler and Steve E. Naranjo
Affiliation:(1) Western Cotton Research Laboratory, USDA-ARS, 4135 E Broadway Road, 85040 Phoenix, AZ, USA
Abstract:The gut contents of field-collected, predaceous Heteroptera were assayed for the presence of eggs of the sweetpotato whitefly,Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) and the pink bollworm,Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) using multiple enzyme-linked immunosorbent assays (ELISAs). Of seven species examined,Geocoris species andOrius tristicolor (Say) were the most frequent predators of sweetpotato whitefly with 32–39% of the individuals tested over the whole season scoring positive for whitefly antigens. With the exception ofLygus hesperus Knight, a major insect pest as well as a predator, the frequency of predation on pink bollworm eggs was much lower (0.7–14.3% positive over the season). Relatively few predators tested positive for both antigens (0.3–12.5%).
Keywords:insecta  predation  ELISA  gut contents
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